Part:BBa_K2685000
lignin peroxidases
Synthetized by Geneart Lignin peroxidase (Lip) is a monomeric haemoglycoprotein secreted by wood-degrading fungi, such as the white wood-rot fungus Phanerochaete chrysosporium.The study of Haemmerli and Hammel in 1986 found that Lignin peroxidase have many substrate,mainly including the non-phenols and phenols aromatic compouds.It can oxidizes lignin monomer, two,three bodies and polycyclic aromatic hydrocarbons.The degradation of the substrate features is that lignin peroxidase can oxidize non-phenols and phenols compounds which rich in electronics.Lignin peroxidase can capture an electronic from the benzene ring when those attacking the substrate by eletron carrier.And then,lip can oxidize the substrate into free rdical and through chain reaction to generate kinds of radical. Those reaction above would lead to the main bond cleavage of the substrte molecule,and then following a series of cracking reaction.
In this year, we use this special peroxidase to degradate the tetracycline in the degradation parts.We also have
optimized this enzyme condon to better express in our constructive chasis.Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 483
Illegal AgeI site found at 460 - 1000COMPATIBLE WITH RFC[1000]
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